Q&A: What percent gel for Western blot should I use?

Depending on the protein size that you’re looking for. To see what protein size that you’re expecting, go to UCSC genome browser to get the protein molecular weight, and also run a molecular weight marker next to your protein lysates.

Protein Size –> Gel percentage

4-40 kDA –> Use 20%

12-45 kDA –> Use 15%

10-70 kDA –> Use 12.5%

15-100 kDA –> Use 10%

25-200 kDA –> Use 8%

Reference: Abcam