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Remove the serum bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes
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Place each container in a 30 to 37°C water bath or incubator. Excessive temperatures will degrade heat-labile nutrients. Prevent the bottle from tilting over.
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Gently swirl or shake the bottles every 10 to 15 minutes until is completely thawed.
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After thawing, use serum promptly. Liquid serum may be stored refrigerated (2 to 8°C) for up to 4 weeks. Ultimately, this length of time where cell culture can be successful will vary with different cell lines. Repeated freeze and thaw series may cause a decrease in serum performance. To avoid thaw/freeze cycles or long periods of refrigeration it is recommended that any unused serum be immediately dispensed into small aliquots and refrozen.
Adapted from: http://www.biowestusa.com/